for laboratory animals, 341
for mice, 342
for rabbits, 343
for rats, 342
Calculated figure for weight of
medium mass, 166, 167
Cambier's candle method of isolating
coli-typhoid groups, 438
Camera lucida, 62
Capaldi-Proskauer medium, No I, 186
No II, 187
Capillary pipettes, 10
graduated, 13
Capitate bacilli, 139
Capsule formation, 134
of bacteria, 134
thermo-regulator, 218
Capsules, collodion, inoculation of, 357
preparation of, 357
glass, 6
to clean infected, 20
new, 18
to stain, 99
to sterilise, 31
Carbohydrate media, preparation of, 177
Carbolic acid as a germicide, 27, 481
method of isolating coli-typhoid group, 437
Carbolised agar, 202
bouillon, 202
gelatine, 202
Carbon dioxide in cultures, test for, 289
Card index, 336, 402
Carrot media, 200
Cedarwood oil for immersion lens, 88
Cell wall of bacteria, 134
Celloidin sacs, manufacture of, 358
Cellular incubator, 216
Centrifugal machine for blood and serum work, 327
for milk work, 447
Centrifugalised milk, 449
Centrigade degrees, conversion of, 494
Chemical products of bacteria, 145
China green agar (Werbitski), 207
Chloroform as an antiseptic, 27
Chromatic aberration, 56
Chromogenic bacteria, 131
Chromoparous bacteria, 144
Chromophorous bacteria, 144
Citrated blood agar, 191
Cladothrix, morphology, 193
Classification of bacteria, 131
of fungi, 126
Clavate bacilli, 139
Clearing films with acetic acid, 82
Clostridium, 139
Coarse adjustment, 51
Cobweb micrometer, 66
Cocaine, 345
Cocci, morphology of, 131
Coccidium infection, 339
Coefficient, inferior lethal, 312
of inhibition, 311
phenol, 489
superior lethal, 313
Cohn's solution, 191
Cold incubator, 217
Coli-typhoid group, differential table, 433
in milk, 451
in soil, 477
isolation of, 432
members of, 430
Collection of blood for bacteriological examination, 378
for media making, 168
of milk samples, 443
of pathological material during life, 373
of pus, 373
of soil sample, 471
of water samples, 416
Collodion capsules, 357
sacs, manufacture of, 357
Colonies of bacteria, edges, 267
Coloured light, action of, 309
Columella, 127
Comparative haemocytology, 374
Complement, definition of, 325
fixation test, 393
Concentration method in water, analysis, 434
Condenser achromatic, 54
dark ground, 60
paraboloid, 60
substage, 54
Condidium, 128
Continuous sterilisation, 36
Contrast stains, 93
Corrosive sublimate (Lang), 82
Cotton-wool filter, 40
Counterstaining films, 84
Counting plate colonies, 423
Cover-slip films, 81
to clean new, 22
used, 24
Crates for test-tubes, 31
Cream, analysis of, 457
qualitative analysis of, 458
quantitative analysis of, 457
Crenothrix morphology, 133
Criteria of infection, 370
Criterion of immunity, 324
Cultural characters, macroscopical examination, 261
Culture flask, Guy's, 5
Kolle, 4
Roux, 5
Cuneate bacilli, 139
Cutaneous inoculation, 352
Dark ground condenser, 60
illumination, 87
Daughter cells, 129
Daylight, diffuse, action of, 308
Decimal scales, 340
Decolourising agents, 84
Definition of objective, 56
Depilatory powder, 346
Description of plate culture, 261
Descriptive terms, 261
Desiccation, effects of, 306
table, 501
Desiccator, Mueller's, 307
Dextrose solution, preparation of, 178
Diaphragm, iris, 53
Diastatic enzymes, tests for, 278
Differential atmosphere cultivation, 257
incubation, 255
media, 255
staining, 108
sterilisation, 256
Diluting chamber, 248
Dilution by teat pipette, 383
of serum, 382
tables, 498
Dilutions, preparations of, 496
Diphtheria, bacillus of, in milk, 452
Diplobacilli, morphology of, 133
Diplococci, morphology of, 133
Diplococcus pneumoniae, immunisation against, 322
Discontinuous sterilisation, 36
Discs of plaster-of-Paris, 192
Disinfectants, action of, 310
chemical, 27
testing of, 480
Dissociating fluid, Price Jones; 400
Dosage of inoculum, 316
Double nosepiece, 58
stains for spores, 106
sugar agar (Russell), 207
Drop-bottle, 73
Dry heat, 28
Dunham's solution, 177
Dyes, aniline, 83
Earthenware box for dirty slides, 70
Earthy salts agar (Lipman and Brown), 197
Edge of individual colonies, characters of, 267
Egg albumin agar, 213
broth, (Lipschuetz), 213
media (Dorset), preparation of, 174
inspissated, 212
(Lubenau), 209
(Tarchanoff and Kolesnikoff), 212
to clear nutrient media with, 166
Ehrlich's eyepiece, 55
Eikonometer, 65
Eisenberg's milk-rice medium, 189
Electric dental engine, 360
signal clock, 38
warm stage, 59
Elevation of colonies, 263
Eisner's gelatine, 204
method of isolating coli: typhoid group, 438
Endogenous spores, 138
varieties of, 139
Endo-germination, 139
English proof agar, Blaxall, 193
Enumerating colonies on plates, 423
discs, Jeffer's, 424
Pakes', 424
Enrichment method in water analysis, 427
Enumeration of micro-organisms, 423
Environmental conditions, 142
Enzyme production, investigation of, 277
Eosin, 93
Equatorial germination, 140
Erlenmeyer flask, 4
Ernstschen Koerner, 136
Esmarch's roll culture, 226
water collecting bottle, 417
Estimation of reaction of media, 280
Ether flame, 28
soluble acids, 284
Eucaine, 345
Exalting virulence of organisms, 320
Examination of milk, 441
Experimental infections, study of, during life, 370
inoculation of animals, 332
Extracellular toxins, 144
Eyepiece, Ehrlich, 55
_micrometer_, 63
Eyepieces, 55
Eye-shade, 57
Fahrenheit degrees, conversion of, 495
Feeding experiments, 369
Fermentation reactions, 279
tubes, 17
Field of objective, 56
Filar micrometer, 66
Filling tubes, etc., with medium, 160
Film preparations, 81
fixing, 81
making, 81
mounting, 85
staining, 83
Filter candle, closed, 47
open, 43
testing efficiency of, 478
to disinfect, 28
to sterilise, 29
flask, 6
papers, to fold, 156
Filters, cotton-wool, 40
porcelain, 42
testing of, 478
Filtration, 40
by aspiration, 42
of media, 156
under pressure, 45
Fine adjustment, 51
spindle head, 52
Fish, analysis of, 460
bouillon, 190
Fish gelatine, 190
gelatine-agar, 190
Fishing colonies, 253
Fission, reproduction by, 136
Fixation, 81
by heat, 81
of tissues, 114
Fixing fluids, for films, 82
Flagella, classification of bacilli by, 136
to stain, 101
Flask Bohemian, 4
Erlenmeyer, 4
filter, 6
Kitasato'a serum, 6
Kolle's culture, 4
Flasks and test tubes, to plug, 24
to clean dirty, 20
new, 18
to sterilise, 31
Fleischwasser, 148
Fluid cultures, description of, 271
media, 146
Foot of microscope, 50
Formaldehyde in milk, Hehner's test for, 442
Formalin method of preserving cultures, 407
tissues, 404
Fractional sterilisation, 33
Fraenkel and Voge's solution, 183
Fraenkel's earth borer, 472
Freezing method for sections, 115
French Mannite Agar (Sabouraud), 193
proof agar (Sabouraud), 193
Fresh preparations of bacteria, 74
Friedlaender's capsule stain for sections, 123
Frost's mounting fluid, 406
Frozen sections, rapid method, 116
Fuchsin, 92
agar (Braun), 205
sulphite agar (Endo), 206
Gas analysis, qualitative, 290
quantitative, 290
collecting apparatus, 291
generators, 242
production by bacteria, 289
tubes for media, 161
Gasperini's solution, 193
Gelatin agar, 193
preparation of, 164
surface plates, 231
General anaesthetics, 345
Gentian violet, 91
German lined paper, 69
Germicides, 27
testing power of, 480
Germination, 140
Geryk air-pump, 43
Glass apparatus in common use, 3
to clean, 18
Glass-cutting knife, 8
Glucose formate agar (Kitasato), 180
bouillon (Kitasato), 180
gelatine (Kitasato), 180
Glycerinated potato, 209
Glycerine agar, 209
blood-serum, 208
bouillon, 209
potato bouillon, 203
broth, 203
Goadby's gelatine, 214
Gonidium, 128
Goniodophore, 128
Graduated capillary pipettes, 13
pipettes, 6
Gram-Claudius' differential stain, 109
Gram's differential stain, 108
Gram-Weigert for sections, 121, 122
Gram-Weigert's differential stain, 109
modified, 110
Grease pencils, 72
Grouping of bacteria for study, 410
Guarded trepine, 360
Guarniari's agar gelatine, 194
Guinea-pig cages, 343
holder, 350
Gulland's solution, 82
Gum solution, preparation of, 116
Guy's culture bottle, 5
Gypsum blocks (Engel and Hansen), 192
Haematin, 95
Haematocytometer, 248
Haematoxilin, 95
Haemolysin, definition of, 326
preparation of, 327
storage of, 331
Haemolytic serum, titration of, 328
Hanging-block culture (Hill), 235
Hanging-drop cultures, 233
examination of, 86, 79
preparation of, 78
permanent staining of, 80
slides, 70
Hardening tissues, 114
Haricot agar, 200
bouillon, 200
Hay infusion, 200
Hearson's water bath, 299
Heat effect of, 299
Hehner's test, 442
Heiman's serum agar, 210
Hesse's anaerobic culture method, 237
Histological examination of blood, 373
Holder for guinea-pigs, 350
Hot air, 29
steriliser, 30
to use, 31
incubator, 217
Hot-water funnel, 158
Human blood agar plates, 250
Huyghenian eyepiece, 55
Hydrogen, generating apparatus, 242
in culture, test for, 289
peroxide in milk, test for, 442
Hyphomycetes, morphology of, 126
reproduction of, 126
Ice-box, for water samples, 419
Ice cream, analysis of, 457
Illuminant for microscope, 67
Immune body, 393
Immunisation, methods of, 321
Imperial system, 492
factors for converting, 493
Impression films, 85
Incubators, 216
Index cards, 336, 403
Indol, test for, 286
Infection, definition of, 370
general observations during life, 371
results of, 404
Influence of environment on bacterial growth, 142
Inhalation, fluid inoculum, 365
powdered inoculum, 366
Inhibition coefficient, 310, 311
Inoculation card index, 336
cutaneous, 352
intracranial, 360
intramuscular, 355
intraocular, 362
intraperitoneal, 355
intrapulmonary, 363
intravenous, 363
of collodion capsules, 357
subcutaneous, 353
syringe, 344
Inoculum, character of, 346
preparation of, 346
Inosite-free media--bouillon (Durham), 183
Inseparate toxins, 144
Intermittent sterilisation, 36
Intracellular toxins, 144
Intracerebral inoculation, 362
Intracranial inoculation, 360
Intragastric inoculation, large animals, 367
Marks method, 367
Intramuscular inoculation, 355
Intraocular inoculation, 362
Intraperitoneal inoculation, 355
Intrapulmonary inoculation, 363
Intravenous inoculation, 363
In vacuo anaerobia cultures, 289
Invertin enzymes, tests for, 279
Involution forms, 137
Iodine solution, 108
Iron bouillon, 185
peptone solution (Pakes), 185
Isolation by animal experiments, 258
by differential atmosphere, 257
incubation, 255
media, 255
sterilisation, 256
by dilution, 248
by plate cultures, 250
subcultures, preparation of, 254
Jeffer's counting disc, 424
Jenner's stain, 97
Jores' mounting fluid, 405
Kaiserling fixing solution, 405
Kanthack's serum agar, 211
Killed cultivations, 318
Kipp's hydrogen apparatus, 242
Kitasato's serum flask, 6
Klebs-Loeffler bacillus in milk, 452
Koch's steam steriliser, 34
Kohle's culture flask, 4
Lab enzymes, test for, 279
Laboratory animals, 335
comparative haematocytology of, 374
normal temperature, 372
regulations, 1
Lactose litmus agar (Wurtz), 203
bouillon, 203
gelatine (Wurtz), 203
Lakmus Molke, 203
Lang's solution, 82
Lead bouillon, 185
peptone solution, 186
Leishman's stain, 98
for sections, 125
Lemco broth, 163
Leptothrix, morphology, 133
Lethal dose, minimal, 316
Leviditi's staining method, 124
Light, action of, 308
Liquefiable media, 147
Liquid soap, 346
Lithium carmine, 96
Litmus bouillon, 186
gelatine, 202
milk cultures, description of, 272
preparation of, 172
nutrose agar (Drigalski-Conradi), 205
whey, 195
agar, 196
gelatine, 196
(Petruschky), 195
Local anaesthetics, 345
reaction to infection, 372
Locomotive movement, 80
Loeffler's capsule stain, 103
serum, 208
Lophotrichous bacilli, 136
Lorrain Smith electric warm stage, 59
serum, 208
Lugol's solution, to prepare, 108
Lysol, 27
MacConkey's capsule stain, 99
media, 180, 199, 205
MacCrorrie's capsule stain, 103
Macroscopical examination of cultures, 261
Malachite green agar (Loeffler), 207
Malt extract solution (Herschell), 196
Margin of individual colonies, 267
Martin's filtering apparatus, 320
Material for inoculation, 346
Mayer's albumin, 120
Mean phenol coefficient, 490
Measuring bacteria, 61
Meat, bacteriological analysis of, 460
extract preparation of, 148
reaction of, 149
Mechanical separation of bacteria, 249
stage, 52
Media, filtration of, 156
preparation of, 163
aerobic culture, 222
aesculin agar, 204
agar-agar, 167
agar gelatine (Guarniari), 194
Media, preparation of anaerobic culture, 180
animal tissue (Frugoni), 210
ascitic bouillon, 210
fluid agar (Wassermann), 213
asparagin (Fraenkel and Voge's), 183
(Uschinsky), 183
beer wort, 175
beetroot, 200
Beyrinck's solution I, 197
II, 198
bile salt agar (MacConkey), 205
broth (MacConkey), 180
double strength, 199
blood agar (Washbourn), 214
blood-serum, 168
(Councilman and Mallory), 208
(Loeffler), 208
(Lorrain Smith), 208
bouillon, 163
bread paste, 193
brilliant green agar (Conradi), 206
bile salt agar (Fawcus), 206
Capaldi-Proskauer, No. I, 186
No. II, 187
carbohydrate, 177
carbolised agar, 202
bouillon, 202
gelatine, 202
carrot, 200
China green agar (Werbitski), 207
citrated blood agar, 171
Cohn's solution, 191
dextrose solution, 178
double sugar agar (Russell), 207
earthy salt agar (Lipman and Brown), 197
egg Dorset, 174
Lubenau, 209
egg-albumen, inspissated, 212
(Tarchanoff and Kolesnikoff), 212
egg-albumin agar, 213
broth (Lipschuetz), 213
English proof agar (Blaxall), 193
fish bouillon, 190
gelatine, 190
agar, 190
fluid, 146
French mannite agar (Sabouraud), 193
Media, preparation of French proof agar (Sabouraud), 193
Fuchsin agar (Braun), 205
sulphite agar (Endo), 206
gelatine, 193
agar, 193
glucose formate agar (Kitasato), 180
bouillon (Kitasato), 180
gelatine (Kitasato), 180
glycerinated broth, 209
potato, 209
glycerine agar, 209
blood-serum, 208, 209
bouillon, 209
potato bouillon, 203
gypsum blocks (Engel and Hansen), 192
haricot agar, 200
bouillon, 200
hay infusion, 200
inosite free-bouillon (Durham), 183
iron bouillon, 185
peptone solution (Pakes), 185
lactose litmus agar (Wurtz), 203
bouillon, 203
gelatine (Wurtz), 203
lakmus molke, 203
lead bouillon, 185
peptone solution, 186
lemco broth, 163
liquefiable, 147
litmus bouillon, 186
gelatine, 202
milk, 172
nutrose agar (Drigalski-Conradi), 205
whey, 195
agar, 196
gelatine, 196
(Petruschky), 195
malachite green agar (Loeffler), 207
malt extract solution (Herschell), 196
milk, 172
rice (Eisenberg), 189
(Soyka), 189
Naegeli's solution, 191
Naehrstoff agar (Hesse and Niedner), 199
neutral litmus solution, 179
nitrate bouillon, 185
peptone solution (Pakes), 186
nutrient, 146
agar-agar, 167
Media, preparation of nutrient bouillon, 163
gelatine, 164
nutrose agar (Eyre), 172
oleic acid agar (Fleming), 201
Omeliansky's nutrient fluid, 189
Parietti's bouillon, 202
parsnip, 200
Pasteur's solution, 191
peptone rosolic acid water, 186
water (Dunham), 177
plaster-of-Paris discs, 192
potato, 174
gelatine (Elsner), 204
(Goadby), 214
proteid free broth (Uschinsky), 183
rosolic acid peptone solutions, 186
serum, bouillon, 210
dextrose water, (Hiss), 188
sugar, (Hiss), 188
water, 170
serum-agar (Heiman), 210
(Kanthack and Stevens), 211
(Libman), 212
(Wertheimer), 211
silicate jelly (Winogradsky), 198
solid, 147
special, 182
stock nutrient, 163
sugar, 177
agar, 185
(dextrose) bouillon, 184
gelatine, 184
sulphindigotate agar, 181
bouillon (Weyl), 181
gelatine (Weyl), 181
tissue (Noguchi), 214
turnip, 200
urine agar, 188
bouillon, 187
gelatine, 187
(Heller), 188
wheat bouillon (Gasperini), 193
whey agar, 195
gelatine, 195
wine must, 192
Winogradsky's solution (for nitric organisms), 198
(for nitrous organisms), 198
wood ash agar, 201
wort agar, 176
gelatine, 176
Media, preparation of yeast water (Pasteur), 191
standardisation of, 154
storage of, in bulk, 159
storing tubes of, 161
sore boxes, 162
titration of, 150
tubing of nutrient, 160
Merismopedia, morphology of, 132
Mesophilic bacteria, 143
pathogenic effects, 315
Metabolic end-products, 145
Metachromatic granules, 136
Metal instruments, to sterilise, 28
Metatrophic bacteria, 131
Methods of cultivation, 221
of identification of bacteria, 259
of inoculation, 352
of isolation, 248
of sterilisation, 26
Methylene-blue, 90
Metric system, 492
factors for converting, 493
Meyer's carmine, 96
Microbes of indication, 426
Micrococci, morphology, 132
Micrococcus, melitensis in milk, 456
Micrometer, filar, 66
net, 63
ocular, 63
stage, 62
Micrometry, methods of, 61
Micron, 61
Microscope, 49
Microscopical examination of bacteria, 86
stained, 88
unstained, 86
observations of cultures, 272
Milk, analysis of, qualitative, 446
quantitative, 444
condensed, analysis of, 444
media, 193
preparation of, 172
rice (Eisenberg), 193
(Soyka), 189
samples, collection of, 443
sedimenting tubes, 449
Minimal lethal dose, 316
Mirror for microscope, 55
Moeller's stain for spores, 107
Moist heat, 32
Molecular movement, 79
Monotrichous bacilli, 136
Motility, examination for, 79
true, 80
Moulds, examination of, 126
for paraffin imbedding, 117, 119
Mounting film preparations, 85
paraffin sections, 119
Mouse cages, 342
holder, 351
scales, 341
Mucor mucedo, 126
Mucorinae, 126
Mueller's desiccator, 307
Muffle furnace, 28
Muirs's capsule stain, 100
flagella stain, 101
Museum preparations of bacteria, 407
of tissues, 404
sealing of, 406
Mycelium, 126
Mycoprotein, 135
Naegeli's solution, 191
Naehrstoff agar (Hesse and Niedner), 199
Naked flame, 28
Neisser's stain modified, 111
Net micrometer, 63
Neutral litmus solution, preparation of, 179
red, 94
Nitrate bouillon, 185
peptone solution (Pakes), 186
Nitric organisms in soil, 478
Nitrosoindol reaction, 287
Nitrous organisms in soil, 477
Normal averages (_t.p.r._), 372
serum, 375
Nosepiece, 57
double, 58
triple, 58
Navy's anaerobic method, 244
jars, 245
Nuclei, to stain, 105
Nucleus of bacteria, 135
Numerical aperture, 56
Nutrient media, 146
Nutrose agar (Eyre), preparation of, 172
Object marker, 61
Objectives, 55
Oblique tube cultures, 223
Ocular micrometer, 63
Oculars, 55
Oese, platinum, 71
Oidium, 128
Oil of garlic, 27
of mustard, 27
Oleic acid agar (Fleming), 201
Omeliansky's nutrient fluid, 189
Operation tables (Eyre's), 352
(Tatin's), 351
Opsonic index, 393
Opsonic index, determination of, 390
Opsonin, 387
Optical characters of colonies, 267
Optimum reaction of medium, determination of, 305
temperature, determination of, 298
Organisms of suppuration, 409
Orsat-Lunge gas apparatus, 292
Orth's carmine, 96
Oxford stain for Actinomyces, 112
Oysters, analysis of, 463
Pakes' counting disc, 424
filter reservoir, 45
Papier chardin, 158
Pappenheim's stain, 111
Paraboloid condenser, 60
Parachromophorous bacteria, 144
Paraffin method for sections, 117
sections, mounting of, 119
to stain, 121
Paratrophic bacteria, 131
Parietti's bouillon, 202
method of isolating coli-typhoid group, 437
Parsnip medium, 200
Passages of virus, 320
Pasteur-Chamberland filter, 42
Pasteur's pipettes, 10
solution, 191
Pathogenesis, investigation of, 315
Pathogenic bacteria, 131
study of, 408
Pediococci, morphology of, 132
Penicillium, 128
Peptone rosolic acid water, 186
water (Dunham), preparation of, 177
Percentage formula, 496
Perchloride of mercury, 27
Perisporaceae, 127
Peritrichous bacilli, 136
Permanent preparations of bacteria, 407
of tissues, 404
Petri's dishes, 6
Phagocytic index, 392
Phenol coefficient, 489
production, test for, 287
Photogenic bacteria, 131, 144
Physiological filter, 156
Picric acid solution, 121
(Spengler's), 112
Picrocarmine, 97
Pigment production, observations on, 288
Pipettes, automatic, 13
blood, 11
capillary, 10
cases for, 7
graduated, 6
capillary, 13
Pasteur's, 10
sedimentation, 16
standard graduated, 7
teat, 10
throttle, 13
to clean infected, 20
new, 18
to sterilise, 31
Piridin method of staining spirochaetes, 124
Pitfield's flagella stain, 103
Plasmolysis, 135
Plaster-of-Paris discs, 192
Plate box, 7
cultures, description of, 261
preparation of, 226
levelling stand, 228
Plates, Petri's, 6
to clean infected, 20
new, 18
to sterilise, 31
Platinum needles, 71
method of mounting, 71
Pleomorphism, 133
Polar germination, 140
granules, 136
Polkoerner, 136
Polychrome blood stains, 97
Pooled serum, 379
Porcelain filter, 42
Berkefeld, 42
Chamberland, 42
Doulton, 42
Post-mortem examination of experimental animals, 396
Potato gelatine (Eisner), 204
(Goadby), 214
medium, preparation of, 174
Potted meat, analysis of, 460
Pouring plates, 227
Preparation of experimental animals, 335
Preservatives in milk, 442
Pressure temperature table, 500
Primary colours, action of, 309
Proteid free broth (Uschinsky), 183
Proteolytic enzymes, tests for, 277
Prototrophic bacteria, 131
Psychrophilic bacteria, 143
pathogenic effects, 315
Pus, collection of, 373
Pyrogallic acid solution, 293
Qualitative analysis of air, 470
of milk, 446
of sewage, 467
of soil, 476
of unsound meat, 462
of water, 426
Quantitative analysis of air, 468
of milk, 444
of sewage, 466
of soil, 473
of unsound meat, 460
Rabbit cages, 343
scabies, treatment of, 338
scales, 340
Raising virulence of organisms, 320
Ramsden's micrometer, 66
Range of medium reaction, measurement of, 305
of temperature, measurement of, 298
Rat cages, 342
Raw milk, Saul's test for, 442
Reaction of medium, 305
optimum, 305
range of, 305
scale, 153
Reduced pressure and temperature table, 501
Reducing agents, production, 389
tests for, 289
Reduction of nitrates, 389
Reichert's thermo-regulator, 218
Relation of bacteria to environment, 142
Removal of material from culture tubes, 74
Rennin enzymes, tests for, 279
Reproduction of bacteria, 136
Resistance glass, 6
to lethal agents, 306
Resting stage of bacteria, 137
Restrictions upon experimental inoculations, 334
Ribbert's capsule stain, 101
Roll cultures, 226
Rosolic acid peptone solution, 186
Rosindol reaction, 286
Roux's anaerobic culture method, 237
culture bottle, 5
Sabouraud's medium, 193
Saccharomyces, morphology of, 129
Safranine, 94
Salicylic acid in milk, test for, 443
Saprogenic bacteria, 131
Sarcinae, morphology of, 132
Saul's test, 442
Scales, decimal, 340
trip, 164
Scalpels, to sterilise, 32, 33
Schallibaum's solution, 121
Scheme for study of bacteria, 259
Schizomycetes, classification of, 131
morphology of, 131
Scissors, to sterilise, 32
Sealing museum jars, 406
Searing iron, 397
Sections, special staining methods for, 121
Sedimentation pipettes, 16
tubes, 9
Selecting objectives, 57
Sensitising red blood cells, 395
Serial cultivations, 251
Serological examination of blood, 378
Serum agar (Heiman), 210
(Kanthack and Stevens), 211
(Libman), 212
plates, 250
(Wertheimer), 211
bouillon, 210
collection of, 379
dextrose water (Hiss), 188
inspissator, 169
sugar media (Hiss), 188
water, preparation of, 170
Sewage, analysis of, qualitative, 467
quantitative, 466
Shake cultivations, 225
description of, 271
Shape of colonies, 262
Shaving experimental animals, 349
Shellfish, analysis of, 463
Silicate jelly (Winogradsky), 198
Single stain for spores, 106
Size of colonies, 262
Slanted tube cultures, 223
Slides, to clean new, 22
used, 23
Smear culture, 224
description of, 268
Soap liquid, 346
Soda solution, storage of stock, 154
Sodium bicarbonate in milk, test for, 443
Soil, analysis of, qualitative, 476
quantitative, 473
collection of samples, 471
Solid media, 147
Soluble toxins, 144
Soyka's milk rice, 189
Spear-headed spatula, 402
Special media, 182
Specific serum, 379
dilution of, 382
Spherical aberration, 55
Spirillum, morphology of, 133
Spirochaeta, morphology of, 133
Spirochaetes in tissues, to stain, 124
Spleen extract, 149
Sporangium, 127
Spore formation, arthrogenous, 138
endogenous, 138
method of, 138, 273
germination, method of, 140, 274
observation of, 140, 273