In its vegetative form the bacillus is killed at 55° C. in 10 minutes.
The spores survive a temperature of 80° C. for 20 minutes, but are
killed at 100° C. in 10 minutes.
When sodium chlorid and potassium nitrate were added to glucose-pork
broth in varying amounts, it was found that 3 per cent of sodium
chlorid or 3 per cent of potassium nitrate was sufficient to inhibit
completely the growth of the bacillus at room temperature (20° to 25°
C.).
While the growth of the bacillus was inhibited by sodium chlorid and
potassium nitrate as just stated, it was found that very much stronger
solutions of the two salts failed to destroy the bacillus. Thus it was
found that the bacillus or its spores retained their vitality after
an exposure of 30 days in a solution containing 23 per cent of sodium
chlorid and 6 per cent of potassium nitrate.