CHAPTER VI

THE PREPARATION OF SOURED MILK IN THE HOUSE

There is no great difficulty in making soured milk at home: the

necessary operations are quite simple, but at the same time they must be

conducted with precision and care, otherwise the results may be

unsatisfactory and disagreeable; there may even sometimes be danger in

badly prepared sour milk. It is always an advantage in such matters to

understand the reason of things, and a few notes on the surrounding

conditions, and what has to be accomplished, may be of assistance to the

would-be experimenter.

The majority of intelligent people are now acquainted with the fact that

the germs of bacteria are to be found everywhere on the surface of the

earth, in air, and in water, and that they are the sole cause of the

decay of all manner of perishable articles.

The distribution is unequal--bacteria are much more plentiful where

there is decaying matter--in dirty houses, sewage, or other contaminated

water, etc. Milk is a splendid food for bacteria, and numerous varieties

multiply in it exceedingly, and many of these are injurious, producing

putrefactive changes which render the milk unwholesome, even poisonous

in some cases. Others are beneficial, and are absolutely necessary for

the souring of milk for making butter or cheese and for the ripening of

the latter. The soured milk which is the subject of this book is the

work of certain lactic-acid-producing bacteria, and the problem we have

before us is to encourage the growth of the latter to the uttermost and

to exclude the others.

As bacterial germs are present in the air and readily sow themselves

into any medium with which they come in contact, the first consideration

is to get good fresh milk which has been as little exposed to the air as

possible. The second is to conduct the experiment where the germs are

fewest, and in cleanly surroundings, far removed from decaying matter

and free from taints and smells.

[Illustration: FIG. 38.--Photo-micrograph of smear of

one-month culture of _Bacillus bulgaricus_. In spite of

its age, the culture is perfectly free from any foreign

organisms, which would otherwise lower its value for the

preparation of soured milk, and might, indeed, make it

directly injurious.]

[Illustration: FIG. 39.--Photo-micrograph of culture of

_Bacillus bulgaricus_ in malt. In cases of acute

enteritis, where milk cannot be supported, the lactic

bacilli may be cultivated in malt, and administered to the

patient in this form, with occasional doses of syrup of

malt, in order to induce a vigorous growth of the lactic

ferments in the body.]

[Illustration: FIG. 40.--Photo-micrograph of smear from

milk that had been allowed to sour spontaneously. Ordinary

market milk is always subject to infection from the air,

milking vessels, manure, etc., and from these sources a

mixed bacterial flora arises, with the result that the

milk exhibits curdling, acidity, gaseous fermentation, or

mould growth, after being stored for a short time. This is

due to the action of sush bacilli, diplococci, yeasts, and

moulds as are shown in this illustration.]

However fresh the milk may be, it will contain useless or injurious

bacteria, and we must get rid of these before attempting to introduce

those whose growth we wish to encourage. This is effected by heat. All

the living bacteria and most of the germs are killed at temperatures

somewhat under the boiling point. Having sterilised the milk in this

way, it is necessary, as far as possible, to prevent the entrance of

fresh germs from the atmosphere, and we therefore let the milk cool down

in covered dishes. When the temperature descends to about 100° F. the

culture of the special bacteria is introduced, the covers are replaced,

and the milk vessels maintained at or near this temperature for twelve

hours, when the soured milk is ready for use. It is not necessary to use

fresh culture every time--a little of the soured milk will take its

place, and this may be repeated as many as fourteen times before it is

necessary to start off again with a fresh culture. A great deal depends

on the care exercised and the freedom of the surroundings from bacterial

germs. Under the best conditions wild germs will gradually accumulate in

the soured milk, but their increase may be greatly delayed by attention

to the precautions mentioned. The ordinary souring of milk for butter

and cheese making is conducted in cool surroundings, as already stated,

because in such conditions the lactic-acid-producing germs increase

relatively faster than the wild germs, and so gain the upper hand, but

in the case of our special soured milk we kill out, practically, all

wild bacteria and germs, and the pure culture having the field to

itself, we can conduct the operation at a higher temperature where the

action of the bacteria is at its maximum, and so obtain the necessary

lactification in the minimum of time.

The appliances for the souring of milk on the domestic scale require

some consideration. We propose to describe the principal forms of

apparatus which have been put on the market for the purpose, and then to

give such suggestions as may assist the ingenious in making apparatus

for themselves. Those who wish for information on the subject of larger

apparatus will find it in the following chapter.

[Illustration: FIG. 41--Photograph of Agar Culture,

inoculated with a lactic powder offered to consumers under

a fancy name. Working on the assumption that the presence

of lactic bacteria is inimical to the growth of septic

organisms, this preparation has been placed on the market.

The plate shows, however an abundance of colonies of

foreign organisms--sporogenous bacilli, _Staphylococcus

pyogenes albus_, and _Staphylococcus pyogenes aureus_.

These organisms are shown in the appended

photo-micrographs, and constitute impurities in the

preparation.]

[Illustration: Spore forming bacillus]

[Illustration: _Staphylococcus pyogenes albus_]

[Illustration: FIG. 42--Photograph of Test Tubes of Sterile

Milk, inoculated with a tablet preparation said to contain

pure cultures. The darker liquefied portion of the

tube-contents is due to digestion of the curd by

proteolytic bacteria, thus indicating impure culture. Pure

cultures of lactic acid bacteria coagulate the milk

uniformly, but do not produce any subsequent change in the

appearance of the culture, even after several months.]

[Illustration: FIG. 43--Photograph of Test Tubes of

Sterile Milk, each tube having been inoculated with a

tablet of a preparation said to contain pure cultures.

Here, again, there is evident peptonisation of the curd,

thus indicating infection by proteolytic ferments.

Cultures from these tubes demonstrated the presence of a

foreign organism. Microscopical examination failed to

disclose the presence of _Bacillus Bulgaricus_.]

[Illustration: FIG. 44--Photograph of Test Tubes of

Sterile Milk, inoculated with a tablet of

"Lactobacilline." In contra-distinction to those shown in

Figs. 42 and 43, these tubes exhibit homogeneous curdling

of the milk without any subsequent digestion or

peptonisation of the coagulum. This is indicative of the

purity of the culture.]

The Society "Le Ferment" of Paris, which has been authorised by

Professor Metchnikoff to prepare and supply to the public his sour milk

culture, provides an apparatus for the treatment of the milk. It is

shown in Fig. 45, which consists of a double box having the intervening

space packed with a non-conducting material. It is provided with a

tight lid. Inside, there is accommodation for two milk vessels, each

with a capacity of about two thirds of a pint. The most difficult thing

in the souring of milk is to maintain the temperature as nearly at 100°

F. as possible while the culture is in action. This result is attained

by filling the vessel in the middle with boiling water. The insulated

walls hinder the escape of heat, and the quantity of boiling water used

is calculated to maintain the temperature steady for the twelve hours of

cultivation; but in cold, frosty weather it is necessary to refill the

central vessel with boiling water in the middle of the period.

[Illustration: FIG. 45]

The milk jars are washed with hot boiled water and turned upside down to

dry. They should not be wiped with a cloth. Boil the milk to be treated

for ten minutes, stirring it to promote evaporation, as it is

advantageous to have it in concentrated form. Cool rapidly to 100° F. by

placing the boiling vessel in cold water, add to each bowl one third of

a tube of the culture in powder form, fill up with the boiled and cooled

milk, stir well and cover. Place the jars in the box and fill the

central vessel with boiling water, shut the lid tight, and do not open

it (unless a fresh charge of boiling water is needed) for ten or twelve

hours, when it will be ready for use. If the liquid culture is used (one

small phial for each bowl) the milk should be cooled to 86° F. instead

of 100° F., as with the powder. The culture is also supplied in tabloid

form. The powder and tabloids keep well, but the liquid can only be

relied on for about two months. The milk prepared as above should be

stored in a cool place, the lids being kept on the bowls. It is good for

about two days, after which it becomes too sour. It can be eaten with

sugar, which not only sweetens it but is beneficial in affording

additional suitable food for the acid-producing bacilli. If fresh milk

cannot be had, condensed, sterilised, or pasteurised milk may be used,

but, of course, fresh milk is best. Condensed milk should be diluted

with two parts of boiling water and then treated like ordinary milk.

An apparatus on similar principles is sold by the Maya Bulgare Company,

Ltd., and is illustrated in Fig. 46.

[Illustration: FIG. 46--Soured Milk Apparatus of the Maya

Bulgare Company, Limited, consisting of an insulated box,

hot-water vessel, and covered vessels containing the milk

and culture. The apparatus consists of cabinet in deal,

Maya Bulgare ferment in 20-dose bottles, Maya Bulgare

ferment in 100-dose bottles, compressed Maya Bulgare

tablets in boxes of 8 tubes, Maya Bulgare caramels in

boxes of 40, reduced milk, china funnels, and

thermometers.]

The box is insulated, but the door is on the side, the hot water vessel

is underneath, and the covered vessels containing the milk and culture

are placed on a shelf above. In the front of the illustration are shown

the various packages in which the liquid and powder cultures are put up.

The procedure is exactly the same as with "Le Ferment" apparatus.

Lactic Ferments, Limited, make use of a different principle to maintain

the temperature steady during the lactifying period. Their apparatus

(Fig. 47) consists of a water vessel mounted on a stand. The milk

vessels (tumblers) are placed in the water, and the temperature

maintained at the proper figure by a small night light burning

underneath. It is recommended that the milk should be placed in an

earthenware jar or jug, which is stood in a pot of water kept boiling

for an hour. After cooling add from three to six previously crushed

tabloids of culture, and stir well with a glass rod which has been

sterilised in boiling water. The milk is then transferred to three

tumblers, which it should fill, and these are put into the water vessel,

the water in which should be at about 100° F., and the night light

started.

[Illustration: FIG. 47]

Messrs. Allen & Hanbury, Limited, also make use of the night light to

maintain the proper temperature during incubation in their "Sauerin"

apparatus (Fig. 48).

No water, however, is placed in the metal container. The procedure is

the same as that already described, and both tablet and liquid pure

cultures are supplied. For children it is recommended that the

incubation should occupy from three to four hours only, in other cases

eight to ten hours. Grated nutmeg, ground cinnamon, or other flavouring,

and cream may be used with the soured milk.

The night light is also employed in the "Veronelle" apparatus of Messrs.

Clay, Paget and Company, Limited (Fig. 49).

[Illustration: FIG. 48.--Messrs. Allen & Hanbury's Soured

Milk Apparatus. They maintain the temperature by means of

a night light, and the culture they use they call

"Sauerin."]

[Illustration: FIG. 49.--Vironelle Apparatus for souring

milk, made by Messrs. Clay, Paget & Company, Limited. The

milk in this case is placed in an earthenware jar, and is

sterilised by placing in a saucepan of water and boiling

it. The culture is added after cooling, the period of

incubation being about six hours.]

The containing vessel is of tin or aluminium, and has two stands, the

high one for hot and the low one for cold weather, as in the latter

case greater heat is needed to maintain the incubating temperature. The

milk is placed in an earthenware jar and is sterilised by placing it in

a saucepan of water and boiling it; continuing the boiling for half an

hour. It is allowed to cool to about 98° F., and placed in the

incubator, culture added, and the lamp lighted, the cover of the

incubator being kept on. The period of incubation is given as six hours.

To prepare the next day's supply a tablespoonful of the soured milk is

retained and used instead of the culture. This may be continued for

fourteen days, when a fresh start with culture is necessary. The soured

milk will keep for thirty-six hours. Capacity, one and one half and two

pints; also a large size for family use.

For the preparation of soured milk on a small scale, one of the various

forms of vacuum flasks now on the market may be used with satisfactory

results. A little cold water must be poured into the flasks, and warm

water added, until, by means of three to four changes, boiling water can

be safely poured in without cracking the flask.

This boiling water must be allowed to remain in for about twenty

minutes, and then replaced by freshly boiled milk that has been cooled,

so that its temperature in the flask is about 105° F. The culture of

lactic organisms should then be added, the opening of the flask plugged

with clean cotton-wool, and the cap screwed on.

In an actual test, the temperature of the milk placed in such a flask

was 105° F., at 7.30 P.M., and had dropped to 93° F. by 9 A.M., the

following day. The milk was curdled, and possessed the normal acid taste

of such cultures.

The different types of apparatus are all quite simple, and it would be

easy to make something at home. Get two round tins, the one less in

diameter by from two to three inches than the other, put one or two

pieces of wood across the bottom inside the larger tin, and fill up the

space between with cotton-wool, which is an excellent non-conductor of

heat. Place the smaller tin centrally inside the larger one. Fix three

or four distance pieces of wood in the space between them, fill up with

cotton-wool, leaving a little space at the top to permit the lid of the

smaller tin to be fixed on. The boiling-water vessel may be a tin

saucepan with a lid, but no handle, and its proper place would be on the

bottom of the inner tin. A tripod stand made of three pieces of wire

bound together, and with legs reaching past the hot-water vessel to the

bottom of the tin, would support a false bottom of tin forming the shelf

on which the vessels of milk to be treated would rest. These might be

tumblers covered with tin lids. To prevent radiation through the lid of

the inner tin, a thick pad or hood of cotton-wool packed between cloth

would be placed over the top. A little experimenting would be necessary

to determine the quantity of boiling water required to maintain the

proper temperature.

The soured milk obtained in the above manner is of the consistency of

ordinary buttermilk; a separation of whey frequently takes place, and

this may be poured off if desired. The taste and flavour should be

pleasantly acid and agreeable, and both are distinctive enough to give a

good idea of the purity of the product. In many places a more

concentrated article is made by boiling down the milk to one half or one

third of its bulk, and then fermenting it in the ordinary way. A kind of

thick pudding is thus obtained, which is highly recommended both as an

agreeable article of food and as a stronger medium for the support of

the germs it is the purpose of the sour milk treatment to introduce

into the digestive system.

The cultures for making soured milk can now be had from all the leading

wholesale and retail chemists, with directions as to the quantities to

be used.

It is very necessary that the milk used should be not only fresh and

good, but also free from chemical preservatives. The effect of these is

to inhibit the growth and development of bacteria, and they have

therefore an injurious influence on the special lactifying germs it is

our object to cultivate. Preservatives are now not nearly so much used

as they were, a few years ago, and there should be no difficulty in

obtaining milk free from them.

For keeping both fresh and soured milk good a small ice-chest is an

excellent device. The germs which are continually dropping into milk

kept in open vessels in the air, even in cleanly surroundings, are

mostly kept out in the close ice-chest, and the low temperature prevents

in a natural way the development of the bacterial germs already in the

milk. For the preservation of all kinds of foods the ice-chest is a

capital investment, not to mention the benefit of having cooled drinks,

etc., as required. They manage things well in this respect in the United

States, where ice is regarded as a necessity in the summer time by even

the poorest people, and is delivered with the same regularity as the

milk.

It is necessary to utter a word of warning as to certain forms in which

the sour milk bacillus is being offered to the public. It is being

compounded with sugar, chocolate, and other articles, and sold in the

form of sweets, etc. There is no reliable proof that these preparations

are valuable. Certainly, sugar is a medium in which the lactifying germs

can live, but the quantity so introduced into the system must be very

small compared with what is obtained from properly fermented milk. It

takes some time, even with strong cultures, to fix the acid-producing

germs in the large bowel where they are wanted, and until proper

evidence is forthcoming that confectionery preparations are efficacious

we would recommend that only the regularly fermented milk be used. It

would save trouble, no doubt, to treat oneself with a few chocolate

creams containing the necessary germs daily, but if the matter is taken

up seriously it will be better to take some pains and stick to methods

the efficacy of which has been demonstrated, leaving the others until

their _bona fides_ has been proved. There are always enterprising firms

who are prepared to simplify things for us, but we must make sure that

their simplifications are warranted.

The Bacillus of Long Life A Manual of the Preparation and Souring of Milk for Dietary Purposes, Together with an Historical Account of the Use of Fermented Milks, from the Earliest Times to the Present Day, and Their Wonderful Effect in the Prolonging of Human Existence · 第 6 / 8 章