OUTLOOK FOR BIOSCIENCE—MAJOR PROBLEMS

The problems undertaken are among the most challenging, if not _the_

most challenging, man faces on the space frontier. These include the

quest for the origin of life, the explanation of life and life

processes, the elucidation of the environment’s role in establishing and

maintaining normal organization in living organisms, the possibility of

extraterrestrial life on other planets—the concern of exobiology. The

greatest promise for their solution lies in advances in biological

theory rather than other avenues of research; therefore, it is fortunate

that the need to solve them has come at a time when developments in

experimental biology are at a high level. In addition, technological

developments in electronics and engineering are providing new and

wonderful instruments for this great exploration into the sources of

life. Many of these have had practical application that has made

possible important advances in medical diagnosis and treatment.

The broad national space goals initially charted by NASA have gone

beyond space flight in near-Earth orbit to lunar and interplanetary

exploration by man and machine. For such missions, more intensive and

comprehensive research in the life sciences is needed. Before manned

voyages for extended periods into deep space will be possible, solutions

must be found for problems such as the development of bioregenerative

life-support systems, communication with nonhuman species, and the

development of new methods for transferring knowledge to the human

brain.

The problems are all of the type that could perhaps be solved by truly

great advances in biological theory, and probably not by any other

avenue.

_References_

[ref.1] _Rea, D. G.:_ The Evidence for Life on Mars. Nature, vol. 200,

1963, p. 114.

[ref.2] _Öpik, E. J.:_ Spectroscopic Evidence of Vegetation on Mars.

Irish Astron. J., vol. 5, 1958, pp. 12-13.

[ref.3] _Kuiper, G. P.:_ Visual Observations of Mars, 1956. Astrophys.

J., vol. 125, 1957, p. 307.

[ref.4] _Föcas, J. H.:_ Seasonal Evolution of the Fine Structure of

the Dark Areas of Mars. Planetary Space Sci., vol. 9, 1962, p.

371.

[ref.5] _Dollfus, A.:_ Interpretation of the Polarization of Light

Reflected by the Different Regions of the Surface of Mars.

Compt. Rend., vol. 233, 1951, p. 467.

[ref.6] _Kuiper, G. P.:_ On the Martian Surface Features. Publ.

Astron. Soc. Pacific, vol. 67, 1955, p. 271.

[ref.7] _Sinton, W. M.:_ Spectroscopic Evidence for Vegetation on

Mars. Astrophys. J., vol. 126, 1957, p. 231.

[ref.8] _Rea, D. G.; Belsky, T.; and Calvin, M.:_ Interpretation of

the 3- to 4-Micron Infrared Spectrum of Mars. Science, vol.

141, 1963, p. 923.

[ref.9] _Rea, D. G.; O’Leary, B. T.; and Sinton, W.:_ Mars and the

Origin of the 3.58- and 3.69-Micron Minima in the Infrared

Spectra. Science, vol. 147, 1965, p. 1286.

[ref.10] _Quimby, F. H.:_ Concepts for Detection of Extraterrestrial

Life. NASA SP-56, 1964.

[ref.11] _Urey, H. C.:_ The Planets. Their Origin and Development. Yale

Univ. Press (New Haven), 1952.

[ref.12] _Miller, S. L.:_ Mechanism of Synthesis of Amino Acids by

Electric Discharge. Biochim. Biophys. Acta, vol. 23, 1957, p.

480.

[ref.13] _Oró, J.:_ Synthesis of Adenine From Ammonium Cyanide.

Biochem. Biophys. Res. Commun., vol. 2, 1960, p. 407.

[ref.14] _Ponnamperuma, C.; Sagan, C.; and Mariner, R.:_ Synthesis of

Adenosine Triphosphate Under Possible Primitive Earth

Conditions. Nature, vol. 199, 1963, p. 222.

[ref.15] _Oparin, A. I.:_ The Origin of Life. Macmillan Co. (New York),

1938.

[ref.16] _Calvin, M.:_ Reduction of Carbon Dioxide in Aqueous Solutions

by Ionizing Radiation. Science, vol. 114, 1951, p. 416.

[ref.17] _Miller, S. L.:_ A Production of Amino Acids Under Possible

Primitive Earth Conditions. Science, vol. 117, 1953, pp.

528-529.

[ref.18] _Oró, J.:_ Studies in Experimental Cosmochemistry. Ann. N. Y.

Acad. Sci., vol. 108, 1963, pp. 464-481.

[ref.19] _Ponnamperuma, C.:_ Chemical Evolution and the Origin of Life.

Nature, vol. 201, 1964, pp. 337-340.

[ref.20] _Bernal, J. D.:_ The Physical Basis of Life. Routledge &

Kegan-Paul (London), 1951.

[ref.21] _Fox, S.:_ Anhydrocopolymerization of Amino Acids Under the

Influence of Hypothetically Primitive Terrestrial Conditions.

Federation Proc., vol. 13, 1954, p. 211.

[ref.22] _Akabori, S.:_ On the Origin of the Fore-Protein. _In_ The

Origin of Life on the Earth. A. I. Oparin et al., eds.,

Pergamon Press (New York), 1955, p. 189.

[ref.23] _Harada, K.; and Fox, S. W.:_ The Thermal Synthesis of Amino

Acids From a Hypothetically Primitive Terrestrial Atmosphere.

_In_ The Origin of Prebiological Systems and Their Molecular

Matrices. Academic Press (New York and London), 1965.

[ref.24] _Oparin, A. I.:_ Biochemical Processes in the Simplest

Structures. _In_ The Origin of Life on the Earth. Pergamon

Press (New York), 1959.

[ref.25] _Fox, S. W.:_ Simulated Natural Experiments in Spontaneous

Organization of Morphological Units for Proteinoid. _In_ The

Origin of Prebiological Systems and of their Molecular

Matrices. Academic Press (New York), 1965.

[ref.26] _Briggs, M. H.; and Mamikunian, G.:_ Organic Constituents of

the Carbonaceous Chondrites. Space Sci. Rev., vol. 1, 1963, p.

647.

[ref.27] _Wilk, H. B.:_ Composition of Some Stony Meteorites. Geochim.

Cosmochim. Acta, vol. 9, 1956, p. 279.

[ref.28] _Briggs, M. H.:_ Organic Extracts of Some Carbonaceous

Meteorites. Life Sciences, vol. 2, 1963, p. 1.

[ref.29] _Mueller, G.:_ Interpretation of the Microstructures of

Carbonaceous Chondrites. Nature, vol. 196, 1962, p. 929.

[ref.30] _Mamikunian, G.; and Briggs, M. H.:_ Some Microstructures of

Complex Morphology Observed in Preparations of Carbonaceous

Choridrites Made Under Sterile Conditions. Nature, vol. 197,

1963, p. 1245.

[ref.31] _Kaplan, L. D.; Munch, G.; and Spinrad, H.:_ An Analysis of

the Spectrum of Mars. Astrophys. J., vol. 139, 1964, p. 1.

[ref.32] _Davis. D. R.; and Libby, W. F.:_ Positive Ion Chemistry.

Science, vol. 144, 1961, p. 991.

[ref.33] _Miller, S. L.:_ Production of Some Organic Compounds Under

Possible Primitive Earth Conditions. J. Am. Chem. Soc., vol.

77, 1955, p. 235.

[ref.34] _Oró, J.:_ Experimental Cosmochemistry. _In_ Current Aspects

of Exobiology. G. Mamikunian and M. H. Briggs, eds., Pergamon

Press (New York), 1965.

[ref.35] _Shapley, H.:_ Of Stars and Men. Beacon Press (Boston), 1958,

pp. 11 and 157.

[ref.36] _Lederberg, J.; and Cowie, D. B.:_ Moondust. Science, vol.

127, 1958, pp. 1473-1475.

[ref.37] CETEX: Development of International Efforts To Avoid

Contamination by Extraterrestrial Exploration. Science, vol.

128, 1958, pp. 887-889.

[ref.38] CETEX: Contamination by Extraterrestrial Exploration. Nature,

vol. 183, 1959, pp. 925-928.

[ref.39] _Sagan, C.:_ Biological Contamination of the Moon. Proc. Natl.

Acad. Sci. U.S., vol. 46, 1960, pp. 393-401.

[ref.40] _Davies, R. W.; and Communizis, M. G.:_ Sterilization of Space

Vehicles To Prevent Extraterrestrial Biological Contamination.

Proc. 10th Intern. Astro. Congr., Springer-Verlag (Vienna),

1960, pp. 495-504.

[ref.41] _Lederberg, J.:_ Exobiology: Approaches to Life Beyond the

Earth. Science, vol. 132, 1960, pp. 393-400.

[ref.42] _Brown, A. H.:_ Report of the Working Subgroup on Space Probe

Sterilization. A Review of Space Research. Natl. Acad.

Sci.-Natl. Res. Council, Publ. 1079, 1962, ch. 10.

[ref.43] _Imshenetskii, A. A.:_ Prospects of the Development of

Exobiology. _In_ Space Research III, W. Priester, ed.,

North-Holland Publ. Co. (Amsterdam), 1963.

[ref.44] _Portner, D. M.; Spiner, D. R.; Hoffman, R. K.; and Phillips,

C. R.:_ Effect of Ultrahigh Vacuum on Viability of

Microorganisms. Science, vol. 134, 1961, p. 2047.

[ref.45] _Morelli, F. A.; Fehlner, F. P.; and Stembridge, C. H.:_

Effects of Ultra-High Vacuum on _Bacillus subtilis_ var.

_niger_. Nature, vol. 196, 1962, pp. 106-107.

[ref.46] _Davis, N. S.; Silverman, G. J.; and Keller, W. H.:_ Combined

Effects of Ultrahigh Vacuum on the Viability of Some Spores

and Soil Organisms. Appl. Microbiol., vol. II, 1963, pp.

202-211.

[ref.47] _Silverman, G. J.; Davis, N. S.; and Keller, W. H.:_ Exposure

of Microorganisms to Simulated Extraterrestrial Space Ecology.

_In_ Life Sciences and Space Research II, M. Florkin and A.

Dollfus, eds., North-Holland Publ. Co. (Amsterdam), 1964, pp.

372-384.

[ref.48] _Imshenetskii, A. A.; Bogrov, N.; and Lysenko, S.:_ Resistance

of Microorganisms to High Vacuum. Doklady Akad. Nauk SSSR,

vol. 154, 1964, pp. 1188-1190.

[ref.49] _Hawrylewicz, E. J.; Gowdy, B.; and Ehrlich, R.:_

Microorganisms Under a Simulated Martian Environment. Nature,

vol. 193, 1962, p. 497.

[ref.50] _Packer, E.; Scher, S.; and Sagan, C.:_ Biological

Contamination of Mars. Part II. Cold and Aridity as

Constraints on the Survival of Terrestrial Microorganisms in

Simulated Martian Environments. Icarus, vol. 2, 1963, pp.

292-316.

[ref.51] _Scher, S.; Packer, E.; and Sagan, C.:_ Biological

Contamination of Mars: I. Survival of Terrestrial

Microorganisms in Simulated Martian Environments. _In_ Space

Research IV. P. Muller, ed., North-Holland Publ. Co.

(Amsterdam), 1964.

[ref.52] _Young, R. S.: Deal, P. H.; Bell, J.; and Allen, J. L.:_

Bacteria Under Simulated Martian Conditions. _In_ Space

Research IV, P. Muller, ed., North-Holland Publ. Co.

(Amsterdam), 1963.

[ref.53] _Hagen, C. A.; Hawrylewicz, E. J.; and Ehrlich, R.:_ Survival

of Microorganisms in a Simulated Martian Environment. Appl.

Microbiol., vol. 12, 1964, pp. 215-218.

[ref.54] _Hawrylewicz, E. J.; Hagen, C. A.; and Ehrlich, R.:_ Response

of Microorganisms to a Simulated Martian Environment. _In_

Life Sciences and Space Research III, M. Florkin, ed.,

North-Holland Publ. Co. (Amsterdam), 1965.

[ref.55] _Nicks, O. W.; and Reynolds, O. E.:_ Decontamination and

Sterilization of Lunar and Planetary Spacecraft. Science, vol.

142, 1963, pp. 539-540.

[ref.56] _Phillips, C. R.; and Hoffman, R. K.:_ Sterilization of

Interplanetary Vehicles. Science, vol. 132, 1960, pp. 991-995.

[ref.57] _Hobby, G.:_ Review of NASA-JPL Spacecraft Sterilization

Program. A Review of Space Research. Natl. Acad. Sci.-Nat.

Res. Council, Publ. 1079, 1962, ch. 10. App. III.

[ref.58] _Bruch, C. W.; Koesterer, M. G.; and Bruch, M. K.:_ Dry-Heat

Sterilization: Its Development and Application to Components

of Exobiological Space Probes. Develop. Ind. Microbiol., vol.

4, 1963, pp. 334-342.

[ref.59] _Koesterer, M. G.:_ Thermal Death Studies on Microbial Spores

and Some Considerations for the Sterilization of Spacecraft

Components. Develop. Ind. Microbiol., vol. 6, 1964, pp.

268-276.

[ref.60] _Hall, L. B.; and Bruch, C. W.:_ Procedures Necessary for the

Prevention of Planetary Contamination. _In_ Life Sciences and

Space Research III, M. Florkin, ed., North-Holland Publ. Co.

(Amsterdam), 1965.

[ref.61] _Portner, D. M.; Hoffman, R. K.; Decker, H. M.; and Phillips,

C. R.:_ The Level of Microbial Contamination in a Clean Room

During a One-Year Period. Rept. 11-65 on NASA Interagency

Agreement R-35, 1964.

[ref.62] _Bruch, C. W.:_ Some Biological and Physical Factors in

Dry-Heat Sterilization: A General Review. _In_ Life Sciences

and Space Research II. M. Florkin and A. Dollfus, eds.,

North-Holland Publ. Co. (Amsterdam), 1964.

[ref.63] _Bruch, C. W.:_ Dry-Heat Sterilization for Planetary-Impacting

Spacecraft. Tech. Rept. on Spacecraft Sterilization. Jet

Propulsion Lab., 1965.

[ref.64] _Pollard, E.:_ Pilot Theoretical Study of the Effect of

Weightlessness and Densely Ionizing Radiation on Single Cells.

Progr. Rept. on Contract NsG-182-62. NASA, 1962.

[ref.65] _Lyon, C. J.:_ Auxin Transport in Leaf Epinasty. Plant

Physiol., vol. 38, 1963, pp. 567-574.

[ref.66] _McKinney, R.; Montgomery, P. O’B.; and Gell, C. F.:_ A Study

of the Effects of Zero Gravity on Cell Physiology. _In_

Physical and Biological Phenomena in a Weightless State.

Second AAS Symp. Phys. Biol. Phenomena Under Zero Gravity

Conditions. E. T. Benedikt and R. W. Halliburton, eds., Advan.

Astronaut. Sci., vol. 14, 1963, pp. 291-306.

[ref.67] Committee on Environmental Biology: Report of Panel on

Gravity. Space Science Board, Natl. Acad. Sci.-Natl. Res.

Council, 1964.

[ref.68] _Chase, H. B.:_ Cutaneous Effects of Primary Cosmic Radiation.

J. Aviation Med., vol. 25, 1954, p. 388.

[ref.69] _Zhukova, A. I.; and Kondratyev, I. I.:_ On Artificial Martian

Conditions Reproduced for Microbiological Investigations. _In_

Life Sciences and Space Research III, M. Florkin, ed.,

North-Holland Publ. Co. (Amsterdam), 1965, pp. 120-126.

[ref.70] _Davis, I.; and Fulton, J. D.:_ Microbiological Studies on

Ecological Considerations of the Martian Environment. USAF SAM

Review, vol. 2, 1959, p. 60.

[ref.71] _Roberts, T. S.; and Irvine, L. A.:_ Studies With a Simulated

Martian Environment. Germination and Growth of Bacterial

Spores. Tech. Doc. Rept. 63-75. USAF School Aerospace Med.,

1963.

[ref.72] _Roberts, T. L.; and Wynn, E. S.:_ Studies With a Simulated

Martian Environment. Bacterial Survival and Soil Moisture

Content. Tech. Doc. Rept. 62-121. USAF School Aerospace Med.,

1962.

[ref.73] _Siegel, S. M.; Giumarro, C.; and Latterall, R.:_ Behavior of

Plants Under Extraterrestrial Conditions: Seed Germination in

Atmospheres Containing Nitrogen Oxides. Proc. Natl. Acad. Sci.

U.S., vol. 52, 1961, pp. 11-13.

[ref.74] _Siegel, S. M.; Halpern, L. A.; Giumarro, C.; Renwick, G.; and

Davis, G.:_ Martian Biology: The Experimentalist’s Approach.

Nature, vol. 193, 1963, pp. 329-331.

[ref.75] _Kiess, C. C.; Corliss, C. H.; and Kiess, H.:_ Evidence for

Oxides of Nitrogen in the Atmosphere of Mars. Science, vol.

131, 1960, p. 1319.

[ref.76] _Vallentyne, J. R.:_ Environmental Biophysics and Microbial

Ubiquity. Ann. N.Y. Acad. Sci., vol. 108, 1963, pp. 342-352.

[ref.77] _Henry, J. P.; Ballinger, E. R.; Maher, P. J.; and Simons, D.

G.:_ Animal Studies of the Subgravity State During Rocket

Flight. J. Aviation Med., vol. 23, 1952, p. 421.

[ref.78] _Van der Wal., F. L.; and Young, W. D.:_ A Preliminary

Experiment With Recoverable Biological Payloads in Ballistic

Rockets. Project MIA. Rept. 715-58, Am. Rocket Soc., 1958.

[ref.79] _Van der Wal, F. L.; and Young, W. D.:_ Project MIA

(Mouse-in-Able) Experiment on Physiological Response to Space

Flight. ARS J., vol. 29, 1954, p. 716.

[ref.80] _Graybiel, A.; Holmes, R. A.; Beischer, D. E.; Champlin, G.

E.; Pedigo, G. P.; Hixson, W. C.; Davis, T. R. A.; Barr, N.

L.; Kistler, W. G.; Niven, J. T.; Wilbarger, E.; Stullken, D.

E,; Augerson, W. S.; Clark, R.; and Berrian, J. H.:_ An

Account of Experiments in Which Two Monkeys Were Recovered

Unharmed After Ballistic Space Flight. Aerospace Med., vol.

30, 1959, p. 871.

[ref.81] _Green, C. D.; Welch, B. E.; Brown, W. L.; Lamb, L. E.; Tang,

P. C.; Gisler, D. B.; and Blodgett, H. C.:_ Studies of Escape

From Ballistic Space Vehicles. Rept. 61-29, School Aviation

Med., sec. 1, pp. 1-24; sec. 2, pp. 1-16, 1961.

[ref.82] _Belleville, R. E.; Rohles, F. H.; Grunzke, M. E.; and Clark,

F. C.:_ Development of a Complex Multiple Schedule in the

Chimpanzee. J. Exp. Anal. Behav., vol. 6, 1963, pp. 549-556.

[ref.83] _Henry, J. P.; and Mosely, J. D.:_ Results of the Project

Mercury Ballistic and Orbital Chimpanzee Flights. NASA SP-39,

1963.

[ref.84] _Grunzke, M. E.:_ A Liquid Dispenser for Primates. J. Exp.

Anal. Behav., vol. 4, 1961, p. 326.

[ref.85] _Grunzke, M. E.:_ Feeding Devices for Use With Primates in

Space Flight. Tech. Doc. Rept. 61-35, USAF Missile Develop.

Center, 1961.

[ref.86] _Gilbert, G. A.:_ A Zero Gravity Pellet Dispenser for Use With

Primates in Long-Term Space Flights. ARL Tech. Rept. 64-15,

1964.

[ref.87] _Meehan, J. P.; Fineg, J.; and Mosely, J. D.:_ The Effect of

Restraint and Training on the Arterial Pressure of the

Immature Chimpanzee. Federation Proc., vol. 23, 1964.

[ref.88] _Findley, J.; and Weissman, N.:_ "Counting" in a Baboon. Paper

presented at Psychonomic Soc. Meeting (New York), 1961.

[ref.89] _Findley, J.; and Brady, J. V.:_ Exposure to Total and

Continuous Environmental Control With a Single Human Organism.

Paper presented at Symp. at 7th Annual Meeting, Human Factors

Soc. (Palo Alto, Calif.), 1963.

[ref.90] _Findley, J.:_ An Experimental Outline for Building and

Exploring Multioperant Behavior Repertories. J. Exp. Anal.

Behav., vol. 5, 1962, pp. 113-116.

[ref.91] _Beasley, J.; and Seldeen, B.:_ The Effect of Prolonged

Acceleration on Eating and Performance. NASA TM X-54:077,

1964.

[ref.92] _Smith, A. H.; Winget, C. M.; and Kelly, C. F.:_ Growth and

Survival of Birds Under Chronic Acceleration. Growth, vol. 23,

1959, p. 97.

[ref.93] _Winget, C. M.; Smith, A. H.; and Kelly, C. F.:_ Effects of

Chronic Acceleration on Induced Nystagmus in the Fowl. J.

Appl. Physiol., vol. 17, 1962, p. 709.

[ref.94] _Wunder, C. C.:_ Food Consumption of Mice During Continual

Centrifugation. Proc. Iowa Acad. Sci., vol. 68, 1961, pp.

616-624.

[ref.95] _Wunder, C. C.:_ Survival of Mice During Chronic

Centrifugation. Aerospace Med., vol. 33, 1962, pp. 866-870.

[ref.96] _Wunder, C. C.; Lutherer, L. C.; and Dodge, C. H.:_ Survival

and Growth of Organisms During Life-Long Exposure to High

Gravity. Aerospace Med., vol. 34, 1963, pp. 5-11.

[ref.97] _Dodge, C. H.; and Wunder, C. C.:_ Growth of Turtles During

Continual Centrifugation. Proc. Iowa Acad. Sci., vol. 69,

1962, pp. 594-599.

[ref.98] _Oyama, J.; and Platt, W.:_ Effects of Deceleration on Rats

Exposed to Prolonged Centrifugation. Nature, vol. 203, 1964,

pp. 766-767.

[ref.99] _Matthews, B. H. C.:_ Adaptation to Centrifugal Acceleration.

J. Physiol., vol. 122, 1953, p. 31.

[ref.100] _Riccio, D.:_ The Effects of Vestibular Stimulation on

Performance Under Several Schedules of Reinforcement. Paper

presented at Eastern Psych. Assoc. Meeting (Atlantic City,

N.J.), 1965.

[ref.101] _Guedry, F. E.; and Biberman, N.:_ Apparent Adaptation Effects

in Vestibular Reactions. Rept. 293, USA Med. Res. Lab., 1957.

[ref.102] _Guedry, F. E.; Cramer, R. L.; and Koella, W. P.:_ Experiments

on the Rate of Development and Rate of Recovery of Apparent

Adaptation Effects in the Vestibular System. Rept. 338. USA

Med. Res. Lab., 1958.

[ref.103] _Guedry, F. E.; and Graybiel, A.:_ The Appearance of

Compensatory Nystagmus in Human Subjects as a Conditional

Response During Adaptation to a Continuously Rotating

Environment. Rept. 531, USA Med. Res. Lab., 1962.

[ref.104] _Loret, B. J.:_ Optimization of Manned Orbital Satellite

Vehicle Design With Respect to Artificial Gravity. ASD Tech.

Rept. 61-688, Wright-Patterson AFB, Ohio, 1961.

[ref.105] _Belleville, R. E.; Clark. F. C.; and Lange, K. O.:_ The

Behavior of Small Animals Under the Accelerative Conditions

Found in Space Travel. NASA TT F-9080, 1964.

[ref.106] _Hebb, D. O.:_ The Organization of Behavior. John Wiley &

Sons, Inc., 1949.

[ref.107] _Jeffress, L. A.:_ Cerebral Mechanisms in Behavior. John Wiley

& Sons, Inc., 1951.

[ref.108] _Konorsky, J.:_ Mechanisms in Animal Behavior. Symp. Soc. Exp.

Biol., vol. 4, Academic Press, 1950.

[ref.109] _Eccles, J. C.:_ The Neurophysiological Basis of Mind. Oxford

Univ. Press (London), 1953.

[ref.110] _Thorpe, W. H.:_ Learning and Instinct in Animals. Methuen

(London), 1956.

[ref.111] _Sperry, R. W.:_ On the Neural Basis of the Conditioned

Response. Brit. J. Anim. Behav., vol. 3, 1955, p. 41.

[ref.112] _Burns, B. D.:_ The Mammalian Cerebral Cortex. Monographs

Physiol. Soc. (London), vol. 5, 1958.

[ref.113] _Krech, D.; Rosenzweig, M. R.; and Bennett, E. L.:_ Dimensions

of Discrimination and Level of Cholinesterase Activity in

Cerebral Cortex of the Rat. J. Comp. Physiol. Psychol., vol.

49, 1956, p. 261.

[ref.114] _Krech, D.; Rosenzweig, M. R.; and Bennett, E. L.:_

Correlation Between Brain Cholinesterase and Brain Weight

Within Two Strains of Rats. Am. J. Physiol., vol. 196, 1959,

pp. 31-32.

[ref.115] _Bennett, E. L.; Rosenzweig, M. R.; Krech, D.; Karlsson, H.;

Dye, N.; and Ohlander, A.:_ Individual, Strain, and Age

Differences in Cholinesterase Activity of the Rat Brain. J.

Neurochem., vol. 3, 1958, p. 153.

[ref.116] _Rosenzweig, M. R.; Krech, D.; and Bennett, E. L.:_ Effect of

Pentobarbital Sodium on Adaptive Behavior in the Rat. Science,

vol. 123, 1956, pp. 371-372.

[ref.117] _Rosenzweig, M. R.; Krech, D.; and Bennett, E. L.:_ A Search

for Relations Between Brain Chemistry and Behavior. Psychol.

Bull., vol. 57, 1960, p. 476.

[ref.118] _Hyden, H.:_ Biochemistry of the Central Nervous System.

Pergamon Press (New York), 1959.

[ref.119] _Hyden, H.; and Egyhazi, E.:_ Nuclear RNA Changes of Nerve

Cells During a Learning Experiment in Rats. Proc. Natl. Acad.

Sci. U.S., vol. 48, 1962, p. 1366.

[ref.120] _Brachet, J.; and Mirsky, A. E.:_ The Cell: Biochemistry,

Physiology, Morphology. Vol. IV, ch. 5. Academic Press (New

York), 1960.

[ref.121] _Tower, D. B.; and Schade, J. P.:_ Structure and Function of

the Cerebral Cortex. Elsevier (New York), 1960.

[ref.122] _Dingman, W.; and Sporn, M. B.:_ The Incorporation of

8-Azaguanine Into Rat Brain RNA and Its Effect on Maze

Learning by the Rat. An Inquiry Into the Biochemical Basis of

Memory. J. Psychiatric Res., vol. 1, 1961, pp. 1-11.

[ref.123] _Adey, W. R.; Kado, R. T.: Didio, J.; and Schindler, W. J.:_

Impedance Changes in Cerebral Tissue Accompanying a Learned

Discriminative Performance in the Cat. Exp. Neurol., vol. 7,

1963, pp. 282-293.

[ref.124] _Brattgard, S. O.:_ Acta Radiol. Suppl., vol. 96, 1952, p. 1.

[ref.125] _Geiger, A.:_ Correlation of Brain Metabolism and Function by

the Use of a Brain Perfusion Method _In Situ_. Physiol. Rev.,

vol. 38, 1958, pp. 1-20.

[ref.126] _Geiger, A.:_ Metabolism of the Nervous System. D. Richter,

ed., Pergamon Press (New York), 1957.

[ref.127] _Thompson, R.; and McConnell, J.:_ Classical Conditioning in

the Planarian _Dugesia dorotocephala_. J. Comp. Physiol.

Psychol., vol. 48, 1955, p. 65.

[ref.128] _McConnell, J. V.; Jacobson, A. L.; and Kimble, D. P.:_

Effects of Regeneration Upon Retention of a Conditioned

Response in the Planarian. J. Comp. Physiol. Psychol., vol.

52, 1959, p. 1.

[ref.129] _Corning, W. C.; and John, E. E.:_ Effects of Ribonuclease on

Retention of a Conditioned Response in Regenerating

Planarians. Science, vol. 134, 1961, p. 1363.

[ref.130] _Gerathewohl, S. J.; and Gernandt, B. E.:_ Physiological and

Behavioral Sciences, Vol. 1, NASA SP-11, 1962, pp. 399-413.

[ref.131] _Von Bekesy, G. J.:_ DC Potentials and Energy Balance of the

Cochlear Partition. Acoust. Soc. Am., vol. 23, 1951, p. 576.

[ref.132] _Trincker, D.:_ Bestandspotentiale in Bogengangssystem des

Meerschweinchens und ihre Anderungen bei Experimentellen

Cupula-Ablenkunges. Arch. Ges. Physiol., Bd. 264, 1957, p.

351.

[ref.133] _Livingston, R. B.:_ Neural Mechanisms of the Auditory and

Vestibular Systems. Ch. 25, G. L. Rasmussen and W. F. Windle,

eds., Charles C Thomas (Springfield, Ill.), 1960.

[ref.134] _Brodal, A.:_ Neural Mechanisms of the Auditory and Vestibular

Systems. G. L. Rasmussen and W. F. Windle, eds., Charles C

Thomas (Springfield, Ill.), 1960.

[ref.135] _Brodal, A.; Pompeiano, O.; and Walberg, F.:_ The Vestibular

Nuclei and Their Connections, Anatomy, and Functional

Correlations. Charles C Thomas (Springfield, Ill.), 1960.

[ref.136] _Carpenter, M. B.:_ Neural Mechanisms of the Auditory and

Vestibular Systems. Ch. 22, G. L. Rasmussen and W. F. Windle,

eds., Charles C Thomas (Springfield, Ill.), 1960.

[ref.137] _Dow, R. S.; and Moruzzi, G.:_ The Physiology and Pathology of

the Cerebellum. Univ. Minn. Press (Minneapolis), 1958.

[ref.138] _Kempinsky, W. H.:_ Cortical Projection of Vestibular and

Facial Nerves in the Cat. J. Neurophysiol., vol. 14, 1951, p.

203.

[ref.139] _Mickle, W. A.; and Ades, H. W.:_ A Composite Sensory

Projection Area in the Cerebral Cortex of the Cat. Am. J.

Physiol., vol. 170, 1952, p. 682.

[ref.140] _Walzl, E. M.; and Mountcastle, V.:_ Projection of Vestibular

Nerve to Cerebral Cortex of the Cat. Am. J. Physiol., vol.

159, 1949, p. 595.

[ref.141] _Anderson, S.; and Gernandt, B. E.:_ Acta Oto-Laryngol.

Suppl., vol. 116, 1954, p. 10.

[ref.142] _Gernandt, B. E.; and Thulin, C. A.:_ Vestibular Mechanisms of

Facilitation and Inhibition of Cord Reflexes. Am. J. Physiol.,

vol. 172, 1953, pp. 653-660.

[ref.143] _Gernandt, B. E.; and Terzuolo, C. A.:_ Effect of Vestibular

Stimulation on Strychnine-Induced Activity of the Spinal Cord.

Am. J. Physiol., vol. 183, 1955, pp. 1-8.

[ref.144] _Gernandt, B. E.; Katsuki, Y.; and Livingston, R. B.:_

Functional Organization of Descending Vestibular Influences.

J. Neurophysiol., vol. 20, 1957, pp. 453-469.

[ref.145] _Gernandt, B. E.; and Gilman, S.:_ Vestibular and

Propriospinal Interactions and Protracted Spinal Inhibition by

Brain Stem Activation. J. Neurophysiol., vol. 23, 1960, pp.

269-287.

[ref.146] _Gernandt, B. E.; and Gilman, S.:_ Interactions Between

Vestibular, Pyramidal and Cortically Evoked Extrapyramidal

Activities. J. Neurophysiol., vol. 23, 1960, pp. 516-533.

[ref.147] _Akert, K.; and Gernandt, B. E.:_ Electroencephalog. Clin.

Neurophysiol., vol. 14, 1962, p. 383.

[ref.148] _Gerathewohl, S. J.; and Ward, J. E.:_ The Physics and

Medicine of the Upper Atmosphere and Space. O. O. Benson and

H. Strughold, eds., John Wiley & Sons, Inc., 1960, ch. 26.

[ref.149] _Gerathewohl, S. J.:_ Personal Experiences During Short

Periods of Weightlessness Reported by Sixteen Subjects.

Astronaut. Acta, vol. 2, 1956, pp. 2O5-217.

[ref.150] _Henry, J. P.; Augerson, W. S.; Belleville, R. E.; Douglas, W.

K.; Grunzke, M. K.; Johnston, R. S.; Laughlin, P. C.; Mosely,

J. D.; Rohles, F. H.; Voas, R. B.; and White, S. C.:_ Effects

of Weightlessness in Ballistic and Orbital Flight. Aerospace

Med., vol. 33, 1962, pp. 1056-1068.

[ref.151] _Dayhoff, M. O.; Lippincott, E. R.; and Eck, R. V.:_

Thermodynamic Equilibria in Prebiological Atmospheres.

Science, vol. 146, 1964, p. 1461.

[ref.152] _Armstrong, G. T.; Furukawa, G. T.; and Hilsenrath, J.:_ A

Survey of Thermodynamic Properties of the Compounds of the

Elements CHNOPS. NBS Rept. 8521, U.S. Dept. Com., 1964.

[ref.153] _White, W. B.; Johnson, S. M.; and Dantzig, G. B.:_ J. Chem.

Phys., vol. 28, 1958, p. 751.

[ref.154] _Jukes, T. H.:_ Present Status of the Amino Acid Code. J. Am.

Dietet. Assoc., vol. 45, 1964, p. 517.

[ref.155] _Nirenberg, M. W.; and Matthaei, J. H.:_ The Dependence of

Cell-Free Protein Synthesis in E. _coli_ upon Naturally

Occurring or Synthetic Polyribonucleotides. Proc. Natl. Acad.

Sci. U.S., vol. 47, 1961, p. 1588.

[ref.156] _Ochoa, S.:_ Chemical Basis of Heredity, the Genetic Code.

Experientia, vol. 20, 1964, p. 57.

[ref.157] _Kiesow, L.:_ The Energy-Transforming Step in

Nitrobacter-Chemosynthesis. Biochem. Z., Bd. 338, 1963, p.

400.

[ref.158] _Kiesow, L.:_ On the Assimilation of Energy From Inorganic

Sources in Autotrophic Forms of Life. Proc. Natl. Acad. Sci.

U.S., vol. 52, 1964, p. 980.

[ref.159] _Clayton, R. K.; and Adler, H. I.:_ Protein Synthesis and

Viability in X-Irradiated _Rhodopseudonomonas spheroides_.

Biochim. Biophys. Acta, vol. 56, 1962, p. 257.

[ref.160] _Pollard, E.; and Vogler, C.:_ Radiation Action on Some

Metabolic Processes in E. _coli_. Radiation Res., vol. 15,

1961, p. 109.

[ref.161] _Novelli, G. D.; Kameyama, T.; and Eisenstadt, J. M.:_ Cold

Spring Harbor Symp. Quant. Biol., vol. 26, 1961, p. 133.

[ref.162] _Kepes, A.:_ Kinetics of Induced Enzyme Synthesis.

Determination of the Mean Life of a Galactosidase-Specific

Messenger RNA. Biochim. Biophys. Acta, vol. 76, 1963, p. 293.

[ref.163] _Fernandez-Moran, H.:_ New Approaches in Correlative Studies

of Biological Ultrastructure by High-Resolution Electron

Microscopy. J. Roy. Microscop. Soc., vol. 83, 1964, p. 183.

[ref.164] _Fernandez Moran, H.:_ Electron Microscope-Medicines Research:

Tool of Unfulfilled Promise. J. Am. Med. Assoc. 189, 1964,

p.31.

[ref.165] _Fernandez Moran, H.:_ Electron Microscope With High Field

Superconducting Solenoid Lenses. Proc. Natl. Acad. Sci. U.S.,

vol.53, 1965, p. 445.

[ref.166] _Deaver, B. S. Jr.; Swedlund, J. B.; and Bradley, H. Jr.:_

Magnetic Properties of Some Macromolecules of Biological

Interest. Final Rept. PHU 4644. Stanford Res. Inst., 1964.

[ref.167] _Sisakyan, N. M.:_ Problems of Space Biology. Vol. I, USSR

Acad. Sci. Publ. House (Moscow) 1962.

[ref.168] _Sisakyan, N. M. and Yazdovskiy V. I._,: Problems of Space

Biology. Vol. III. USSR Acad. Sci. Publ. House (Moscow),

1964.

[ref.169] _Jenkins D. W._,: The NASA Biosatellite Program. _In_ Life

Sciences and Space Research III, M. Florkin, ed,.

North-Holland Publ. Co. (Amsterdam), 1965, pp. 230-240.

[ref.170] _Pack N.:_ The Effects of Weightlessness on Mammals. Space

Biology: Proc. 24th Annual Biol. Colloq., Oregon St. Univ.,

1963, pp. 65-74.

[ref.171] _Anon.:_ A Review of Space Research. Ch. 9, Natl. Acad.

Sci.-Natl. Res. Council. Publ. 1079, 1962.

[ref.172] Committee on Environmental Biology: Report of Panel on

Radiation Biology. Space Science Board, Natl. Acad. Sci.-Natl.

Res. Council, 1963.

[ref.173] _Taleot, J. M.:_ Life Support in Space Operations. Air Univ.

Rev., vol. 16, 1965, pp. 42-52

[ref.174] _Del Duca, M. G.; Konecci, E. B.; and Ingelfinger A. L.:_ Life

Support-the Next Generation. Space Aeronautics, vol. 41, 1964,

pp. 84-91.

[ref.175] _Bongers. L.; and Kok B.:_ Life Support for Space Missions.

Develop. Ind. Micro-biol., vol. 5, 1964, pp. 183-195.

[ref.176] _Ward, C. H.; Wilks. S. S.; and Craft, H. L.;_ Use of Algae

and Other Plants in the Development of Life Support Systems.

Am. Biol. Teacher, vol. 25, 1963, pp. 512-521.

[ref.177] _Ney L. F.:_ Gas Exchange by the Duckweed Family. ONR Contract

No. Nonr-2887(00), Stanford Res. Inst., 1960.

[ref.178] _Nakamura, H.:_ A Study of Wolffia as a New Food. Rept.

Microalgal Res. Inst. Japan., vol. 1, 1960, pp. 7-13.

[ref.179] _Myers, J.:_ Basic Remarks on the Use of Plants as Biological

Gas Exchangers in a Closed System. _In_ Epitome of Space

Medicine. J. Aviation Med., vol. 25, 1954, pp. 407-411.

[ref.180] _Bowman, N. J.:_ The Food and Atmosphere Control Problems on

Space Vessels. J. Brit. Interplanet. Soc, vol. 12, 1953, pp.

118-123.

[ref.181] _Bowman, N. J.:_ The Food and Atmosphere Control Problems on

Space Vessels. Part II. The Use of Algae for Food and

Atmosphere Control. J. Brit. Interplanet. Soc. Vol. 12, 1953,

pp. 159-166.

[ref.182] _Bassham, J. A.:_ Experiments With Photosynthetic Gas

Exchangers. Proc. World Symp. Appl. Solar Energy, Phoenix,

Ariz., 1955, pp. 35-50.

[ref.183] _Burk D.; Hobby, G.; and Gaucher, T.:_ Closed-Cycle Air

Purification With Algae. Proc. First Intern. Symp. Submarine

Space Med. Macmillan Co. (New York), 1958.

[ref.184] _Dole, S. H.; and Tamplin, A. R.:_ The Sabatier Reaction for

Inorganic Recovery of Oxygen in Manned Space Capsules. _In_

Closed Circuit Respiratory Systems Symp. Tech. Rept. 60-574,

Wright Air Develop. Div., 1960, pp. 239-287.

[ref.185] _Clifford, J. E.; and McCallum, J.:_ Research on the

Electrolysis of Water Under Weightless Conditions. AMRL-Tech.

Doc. Rept. 62-44, 1962.

[ref.186] _Clifford, J.; and Faust, C.:_ Research on Electrolysis of

Water With a Hydrogen Diffusion Cathode To Be Used in a

Rotating Cell. AMRL-Tech. Doc. Rept. 62-94, 1962.

[ref.187] _Rupaske, R.:_ Nutritional Requirements for _Hydrogenomonas

eutropha_. J. Bacteriol, vol. 83, 1962, pp. 418-422

[ref.188] _Bongers, L.:_ Chemosynthetic Gas Exchanger. Quarterly

Progress Rept. No. 4 on NASA Contract NASw-971, 1965, 23 pp.

[ref.189] _Bongers, L.:_ Chemosynthetic Gas Exchanger. Ann. Rept. on

NASA Contract NASw-713, 1964, 44 pp.

[ref.190] _Bongers, L.:_ Chemosynthetic Gas Exchanger. Rept. RM

153-1963. Martin-Marietta Space Systems Div. (Baltimore, Md.),

1963

[ref.191] _Bongers, L.:_ Sustaining Life in Space—A New Approach.

Aerospace Med., vol. 35, 1964, pp. 139-144.

[ref.192] _Schlegel, H.G.:_ Die Verwendung von H₂-oxydierenden Bakterien

zur Regenerierung der Atemluft. Raumfahrtforschung, Bd. 8. Nr.

2, 1964, pp. 65-67.

[ref.193] _Webb. P., ed.:_ Bioastronautics Data Book. NASA SP-3006,

1964.

[ref.194] _Foster. J. F.; and Litchfield, J. H.:_ A Continuous Culture

Apparatus for the Microbial Utilization of Hydrogen Produced

by Electrolysis of Water in Closed-Cycle Space Systems.

Biotech. Bioeng., vol. 6, 1964, pp. 441-456.

[ref.195] _Callaway. D.; and Margen, S.:_ Nutritional Properties of

_Hydrogenomonas eutropha_. Semiann. Progr. Rept., NASA Grant

NGR-05-003-(089), 1965.

[ref.196] Working Group on Gaseous Environment for Manned Spacecraft:

Summary Report. Space Science Board. NAS/NRC, 1963.

[ref.197] _Lawton, R. W.:_ Physiological Considerations Relevant to the

Problem of Prolonged Weightlessness. Astronaut. Set. Rev.,

vol. 4, 1962, pp. 1-16.

[ref.198] _McCally, M.; and Lawton. R. W.:_ The Pathophysiology of

Disuse and the Problem of Prolonged Weightlessness: A Review.

AMRL-Tech. Doc. Rept. 63-3, Aerospace Med. Div.

(Wright-Patterson AFB, Ohio), 1963.

[ref.199] _Smith, R. E.; and Hock, R. J.:_ Brown Fat: Thermogenic

Effector of Arousal in Hibernators. Science, vol. 140, 1963,

p. 199.

[ref.200] _Storer. J. B.; and Hempelman L. H.:_ Hypothermia and

Increased Survival Rate of Infant Mice Irradiated With X-rays.

Am. J. Physiol., vol. 171, 1952, pp. 341-348.

[ref.201] Working Group on Nutrition and Feeding Problems: Summary

Report. Space Science Board. Man-in-Space Comm., NAS/NRC,

1963.

[ref.202] _Anon.:_ Conference on Nutrition in Space and Related Waste

Problems. NASA SP-70, 1964.

* U.S. GOVERNMENT PRINTING OFFICE 1966 - O - 798 520

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