Experiments were performed on five cats which received from 140 to 155
mg per kilo, as follows:
_Cat 24. Striped. Weight, 1,300 grams._
October 25: 10 a. m., 50 cc urine, albumin moderate amount--no sugar;
10 cc caffein injected subcutaneously at 12 noon; 12.30, irritable,
cried persistently, no appetite; 4 p. m., no convulsions, but
persistent crying.
October 27: Cat was still alive.
_Cat 17. Weight, 2,620 grams._
October 12: 9.30 a. m., 65 cc urine collected; more than a trace of
albumin present, no reduction of Fehling's solution; 3 p. m., 20 cc 2
per cent caffein (150 mg per kilo) injected subcutaneously; 3.15 p. m.,
irritable and restless.
October 13: 9 a. m., about 15 cc urine collected, reduction of
Fehling's solution marked; osazone test also positive.
_Cat 23. Black and white. Weight, 1,645 grams._
October 25: 10 a. m., 140 cc urine collected (since October 23), small
amount of albumin present, no sugar.
October 27: 9 a. m., no albumin; no sugar in urine; 11.50 a. m., 12.5
cc caffein injected subcutaneously (0.15 gram per kilo); 1 p. m.,
convulsions and death.
_Cat 7. Striped kitten. Weight, 1,285 grams._
October 11: Urine collected, no albumin, no sugar; 9.50 a. m., 10 cc 2
per cent caffein injected subcutaneously in the back; 10.10, violent
convulsions lasting about 30 seconds; 10.20, convulsions of shorter
duration; 10.30 convulsions; 10.35, convulsions lasting a few seconds;
urine passed about 10.20, contained a moderate amount of albumin, but
there was no reduction of Fehling's solution; 10.45, profuse salivation
and paralysis; died about 10.50.
_Cat 39. Yellow. Weight, 2,285 grams._
April 13: 2.40 p. m., 16 cc 2 per cent caffein (0.14 gram per kilo)
injected subcutaneously in the back; 3.45 p. m., cat died.
Of the five experiments of this series three died after doses of 140,
150, and 155 mg per kilo. The other two showed symptoms of toxicity,
but survived a dose of 150 mg per kilo which indicated that the minimum
fatal dose was probably reached. To test this supposition smaller doses
were administered, as shown in the following experiments.