In the fall of the same year additional experiments were carried out
with doses ranging from 100 to 200 milligrams of caffein per kilo,
which were injected into the lumbar muscles. The results are given in
the following abbreviated protocols:
_Rabbit 425. Belgian hare. Weight 1,520 grams._
September 27: 10.30 a. m., 7.5 cc 2 per cent caffein injected into the
lumbar muscles; 2 p. m., reflexes increased.
September 28: Rabbit normal.
October 5: Weight, 1,620 grams; 2.50 p. m., 10 cc 2 per cent caffein
injected into lumbar muscles; 3.05 p. m., reflexes increased.
October 13: Weight, 1,520 grams; 10.30 a. m., 10 cc 2 per cent caffein
(131 mg per kilo) injected; 11 a. m., no symptoms; 11.30 a. m.,
reflexes much increased.
October 14: Alive, no symptoms.
_Rabbit 426. Belgian hare, female. Weight, 1,425 grams._
September 27: 7 cc 2 per cent caffein injected into the lumbar muscles
at 10.30 a. m.; 2 p. m., reflexes increased.
September 28: Rabbit normal.
October 5: Weight, 1,425 grams; 2.55 p. m., 9 cc 2 per cent caffein
injected into lumbar muscles; 3.05 p. m., reflexes increased.
October 13: Weight, 1,405 grams; 10.30 a. m., 10 cc 2 per cent caffein
(142 mg per kilo) injected; 11 a. m., no symptoms; 11.30 a. m.,
reflexes increased.
October 14: Rabbit alive, no symptoms.
_Rabbit 427. Belgian hare, female. Weight, 1,780 grams._
September 27: 9 cc 2 per cent caffein injected into the lumbar muscles;
2 p. m., reflexes increased.
September 28: Rabbit normal.
October 5: Weight, 1,850 grams; 3 p. m., 11.5 cc 2 per cent caffein
injected into lumbar muscles; 3.10 p. m., reflexes increased.
October 13: Weight, 1,830 grams; 10.40 a. m., 14 cc 2 per cent caffein
(153 mg per kilo) injected into lumbar muscles; 11 a. m., no symptoms;
11.30 a. m., reflexes increased.
October 14: Rabbit alive, no symptoms.
_Rabbit 453. Belgian hare, male. Weight, 1,160 grams._
October 12: 3.45 p. m., 11.5 cc 2 per cent caffein in aqueous solution
injected into lumbar muscles; 4.15 p. m., reflexes increased;
4.30 p. m., paralyzed.
October 13: 9 a. m., found dead. _Autopsy_: Gastric mucosa
hemorrhagic; liver darkened; other organs normal.
_Rabbit 455. Belgian hare, gray, female. Weight, 1,185 grams._
October 12: 3.30 p. m., 11.5 cc 2 per cent caffein injected into the
lumbar muscles; 4 p. m., reflexes increased.
October 13: Rabbit weighed 1,070 grams, no symptom of caffein
poisoning, reflexes normal; 10.30 a. m., 10 cc 2 per cent caffein
injected into the lumbar muscles; 11.30 a. m., jumped off the table,
had attack of convulsions and died. _Autopsy_: Findings same as in No.
453.
_Rabbit 428. Belgian hare, gray, male. Weight, 1,650 grams._
October 5: 4 p. m., 14.8 cc 2 per cent caffein (0.18 gram per kilo)
injected into the lumbar muscles.
October 6: Found dead.
_Rabbit 429. Belgian hare, male. Weight, 1,340 grams._
October 5: 4 p. m., 13.5 cc 2 per cent caffein (0.2 gram per kilo)
injected into lumbar muscles.
October 8: Rabbit found dead.