The experiments of this series were made to determine the minimum toxic
dose.
_Rabbit 295. Belgian hare, female. Weight, 1,205 grams. Diet,
carrots._
March 1: 10.40 a. m., 6 cc 2 per cent caffein (0.1 gram per kilo)
injected into peritoneal cavity; about 2 cc of urine obtained before
injecting caffein; 2 p. m. 100 cc urine, bloody in appearance,
collected, a moderate quantity of albumen present, no reduction; 3.40
p. m., no symptoms.
March 4: 2 p. m., rabbit looks well.
_Rabbit 293. Belgian hare, female. Weight, 1,605 grams. Diet, carrots._
March 1: Urine from bladder clear, alkaline; 11.55 a. m., 8 cc 2 per
cent caffein (0.1 gram per kilo) injected into peritoneal cavity;
3 p. m., 90 cc urine normal in color collected, no albumen, no
reduction; 3.40 p. m., no symptoms.
March 4: 1.15 p. m., rabbit looks normal.
_Rabbit 292. Belgian hare, male. Weight, 1,595 grams. Diet, carrots._
March 1: 10.10 a. m., 8 cc 2 per cent caffein (0.1 gram per kilo)
solution injected into peritoneal cavity; 10.40 a. m., rabbit urinated,
reflexes increased, but no other symptoms; 10.50 a. m., no urine
obtained from bladder; 2 p. m., 105 cc of clear pale urine collected;
no albumen, no reduction; 3.40 p. m., no symptoms.
March 4: 2 p. m., rabbit looks well, urine collected, did not contain
sugar.
_Rabbit 298. Belgian hare, female. Weight, 1,205 grams. Diet, carrots._
March 1: 4.06 p. m., 7.5 cc 2 per cent caffein solution (0.125 gram
per kilo) injected into peritoneal cavity, urine obtained from bladder
immediately after injection, no albumen, no reduction; 5.30 p. m.,
reflexes increased, rabbit was able to run around, but became paralyzed
soon; 5.40 p. m., rabbit is again able to run around.
March 3: 10 a. m., anterior extremities paralyzed, is able to use
posterior extremities.
March 4: 1 p. m., rabbit looks normal.
_Rabbit 223. Belgian hare, male. Weight, 1,165 grams. Diet, carrots._
March 1: 3.50 p. m., urine obtained from bladder clear, amber colored,
no albumen, no sugar; 3.52 p. m., 7.5 cc 2 per cent caffein (125 mg per
kilo) injected into peritoneal cavity; 5.40 p. m., rabbit makes little
attempt to run when put on the floor, weakness of extremities marked.
March 4: 1.15 p. m., rabbit normal.