Wind several yards of thread into coils. Boil for 30 minutes in a 1 per
cent. solution of bicarbonate of soda. Wash in fresh cold water, and
allow to stand in cold distilled water for 6 hours. Then boil in fresh
water for 30 minutes. Place in absolute alcohol for 48 hours, then in a
solution of Schering’s celloidin with equal parts of alcohol and ether.
The mixture must stand for 12 hours, or until dissolved; keep tightly
corked. When ready for use add 1 per cent. of sterile oleum ricini, and
immerse thread for 48 hours. Then wind upon a frame to dry. This will
require from 1 to 3 days. When dry, cut into lengths and coil or wind
on glass slides. Boil for 1 hour in normal salt solution. Preserve in
Chinesol solution, 1: 500.
=To Sterilize Glycerin or Any Kind of Oil.=—Place bottle uncorked in a
vessel of boiling water for 2 hours.